Concentrations of Nitrous Oxide in Blood, Exhaled Air and Urine After Various Types of Exposure.

Recruiting now · Not applicable

Conditions studied: Intoxication by Drug

In brief

The study examines the concentrations of nitrous oxide that are achieved in blood, exhaled air, and urine after three different exposures to nitrous oxide: inhalation of three or four cartridges of nitrous oxide released in a car cabin in a switched-off car, consumption of 100 g of whipped cream in which nitrous oxide is used as a propellant, and exposure through work as a midwife in a delivery room. The concentrations in blood and exhaled air are measured for up to 1 hour after exposure, and in urine for up to 2 hours after exposure. The concentrations in blood and urine are measued with gas chromatography and mass spectrometry, while the concentration in exhaled air is measured by a sensor using an NDIR sensor (Olythe, France).

Key facts

Study ID

NCT07690358

Run by

University of Aarhus

People needed

40

Starts

2026-06-01

Expected to finish

2026-12-31

Last updated by the study team

2026-07-08

Who can join

Age: 18 and older, up to 65. Sex: any. Healthy volunteers: accepted.

You may qualify if…

• Age 18-65 years
• Able to understand Danish
• Willing to use effective contraception for 24 hours after the end of exposure (oral contraceptives, contraceptive implant, transdermal patch, vaginal ring, or injectable contraceptives)
• Written informed consent

You may not qualify if…

• Current or past abuse of drugs, nitrous oxide (N₂O), or alcohol
• A psychiatric disorder requiring medical treatment, including severe depression
• A diagnosis of epilepsy
• Symptoms of peripheral neuropathy
• Significant cardiac or pulmonary disease
• Previous allergic or adverse reaction to N₂O
• Diving within the past 2 days
• Inability to refrain from diving for 2 days after the study
• Occupational exposure to N₂O
• Use of N₂O within the past 3 months
• Treatment with methotrexate, benzodiazepines, opioids, or other centrally depressant drugs
• Recent intraocular gas injection
• Known impaired liver or kidney function
• Pregnant or breastfeeding women (fertile women, defined as amenorrhea for less than 12 months, must have a negative hCG pregnancy test prior to receiving the study intervention)

Where it is running

• Department of Forensic Medicine — Aarhus, Denmark (enrolling)

Full record on ClinicalTrials.gov

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