Metagenomic Analysis and Postoperative Pain in Infected Root Canals Using Different Irrigation Protocols

Starting soon · Not applicable

Conditions studied: Dental Pulp Necrosis, Pulpitis

In brief

Achieving effective root canal disinfection while minimizing postoperative pain is a critical goal in endodontic therapy. Advanced irrigation protocols, such as ultrasonic activation, laser activation, negative pressure irrigation (PulpSucker device), and mechanical activation (Easy Clean device), have shown promise in enhancing bacterial reduction within the root canal system. However, their impact on postoperative pain has not been fully explored. This randomized controlled clinical trial aims to compare bacterial reduction and postoperative pain levels in infected root canals using four different irrigation protocols. The study will use metagenomic analysis to evaluate changes in bacterial community composition and the Visual Analogue Scale (VAS) to assess patient-reported pain levels at multiple time points postoperatively.

Key facts

Study ID

NCT07546773

Run by

Suez Canal University

People needed

40

Starts

2026-04-30

Expected to finish

2028-01-15

Last updated by the study team

2026-05-04

Who can join

Age: 18 and older, up to 60. Sex: any. Healthy volunteers: not accepted.

You may qualify if…

• Adult patients aged 18-60 years
• Requiring root canal treatment for teeth with necrotic pulp
• Presence of periapical radiolucency confirmed radiographically
• Good general health with no systemic diseases affecting healing outcomes
• Willing to provide informed written consent
• Available for follow-up appointments

You may not qualify if…

• Patients with open apices
• Severe periodontal disease
• Currently taking antibiotics
• Allergy to study materials (lidocaine, sodium hypochlorite, methylene blue, latex)
• Pregnant or breastfeeding women
• Compromised immunity or systemic conditions (e.g., uncontrolled diabetes, HIV, immunosuppressive therapy)
• Previous root canal treatment on the study tooth
• Tooth not restorable
• Presence of vertical root fracture

Where it is running

• Endodontic Clinic, Faculty of Dentistry, Suez Canal University — Ismailia, Ismailia Governorate, Egypt

Full record on ClinicalTrials.gov

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