Effect of PRP in Donor Semen During Cryoprervation

Recruiting now

Conditions studied: Sperm Cryodamage

In brief

Sperm cryopreservation allows the preservation of sperm biological function for long periods of time for later use in assisted reproductive techniques (ART). However, the freezing and thawing process causes structural and functional damage to sperm, leading to decreased motility and vitality, a loss of plasma membrane integrity and even DNA damage. In recent years, various strategies have been studied to protect sperm from cryodamage, including the optimization of freezing processes, the design of freezing devices, and improved freezing media. One possible measure to mitigate the cryodamage suffered by sperm is the addition of PRP, or platelet-rich plasma, to the freezing medium. In previous studies, PRP components have been shown to have positive effects on semen quality, improving motility, viability, and plasma membrane integrity after cryopreservation. In light of these positive results, this study will evaluate the possibility of incorporating PRP into sperm cryopreservation media and its potential future benefits for patients and clinical outcomes. To this end, each semen sample after a previous spermiogram will be aliquoted, and 0%, 1%, 2%, and 5% PRP will be added to the cryopreservation medium according to the sperm group to which the belong. A spermiogram will be performed after cryopreservation to assess various semen quality parameters, including sperm count, motility, morphology, vitality, and chromatin dispersion. The data must be processed through statistical analysis to obtain results that allow determining, primarily, whether there is a beneficial effect on sperm quality after sperm motility and sperm during cryopreservation with the addition of PRP.

Key facts

Study ID

NCT07504575

Run by

Instituto Valenciano de Infertilidad, IVI Alicante

People needed

20

Starts

2026-02-10

Expected to finish

2028-01-30

Last updated by the study team

2026-04-17

Who can join

Age: 18 and older, up to 35. Sex: male. Healthy volunteers: accepted.

You may qualify if…

• Age from 18 to 35 years
• Concentration higher than 50 million progressively motile sperm.
• Morphology: more than 4% normal sperm shapes.

You may not qualify if…

• Presence of personal or hereditary pathology.
• Presence of psychological or behavioral disorder.
• Semen parameters below those indicated in the inclusion criteria.

Where it is running

• IVI Alicante — Alicante, Alicante, Spain (enrolling)

Full record on ClinicalTrials.gov

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