Antibiofilm Activity of Chitosan Nanoparticles Against Uropathogenic Escherichia Coli

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Conditions studied: Urinary Tract Infections (UTIs), Catheter-Associated Urinary Tract Infections (CAUTIs), Biofilm Formation by Uropathogenic Escherichia Coli (UPEC)

In brief

1. Isolation of uropathogenic Escherichia coli (UPEC) and determination of their Antimicrobial sensitivity Patterns. 2. Evaluation of biofilm-forming capacity of UPEC isolates. 3. Assessment of the antibiofilm efficacy of chitosan nanoparticles alone and in combination with ciprofloxacin against UPEC isolates. 4. Examination of the effectiveness of chitosan nanoparticle coating in preventing biofilm formation by UPEC on urinary catheter surfaces. 5. Evaluation of the impact of chitosan nanoparticles on the expression levels of biofilm associated genes in UPEC.

Key facts

Study ID

NCT07482553

Run by

Assiut University

People needed

60

Starts

2026-05-01

Expected to finish

2027-06-01

Last updated by the study team

2026-03-27

Who can join

Age: 18 and older. Sex: any. Healthy volunteers: not accepted.

You may qualify if…

• 1. Patients (inpatients or outpatients) clinically diagnosed with urinary tract infection (UTI).
• Presence of significant bacteriuria in urine culture (≥10⁵ colony-forming units per milliliter (CFU/mL) for midstream urine, or as clinically indicated).
• Isolation of Escherichia coli as the sole or predominant pathogen from urine culture.
• Patients of any age and both sexes. 5. Patients who have not received antibiotic therapy within the previous 48-72 hours.

You may not qualify if…

• 1. Patients who received systemic antibiotic therapy within the previous 48-72 hours prior to urine sample collection.
• Urine cultures showing insignificant bacteriuria (<10⁵ CFU/mL for midstream urine, unless clinically justified).
• Polymicrobial growth in urine culture (mixed bacterial growth suggestive of contamination).
• Isolation of organisms other than Escherichia coli. 5. Improperly collected, leaking, or contaminated urine samples. 6. Patients unwilling to provide informed consent.

Where it is running

• Faculty of Medicine, Assiut University, Microbiology and Immunity department — Asyut, Assiut Governorate, Egypt

Full record on ClinicalTrials.gov

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