Impact of Siderophore Genes on Cefiderocol Susceptibility in Carbapenem Resistant Hypervirulent Klebsiella Pneumoniae
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Conditions studied: Carbapenem-resistant Hypervirulent Klebsiella Pneumoniae Infection
In brief
1. Sample: Clinical specimens (blood, urine, sputum, wound swabs) will be collected aseptically and transported to the microbiology laboratory for analysis. 2. Culture: Specimens will be inoculated on MacConkey agar and incubated at 35-37°C for 18-24 hours. Colonies with typical K. pneumoniae morphology (mucoid, lactose-fermenting) will be selected for further testing 3. Identification of isolates will be done by: Colony morphology, Gram staining, and conventional biochemical tests (eg, indole, citrate utilization, urease, Voges-Proskauer) . 4. Antimicrobial susceptibility testing of the isolates : Antimicrobial susceptibility testing was performed and interpreted according to the Clinical and Laboratory Standards Institute (CLSI) guidelines, M100 document (latest edition)." 5. MIC determination for cifederecol using E-test . 6. Phenotypic Detection of hvKP: 1. String Test: A viscous string ≥5 mm formed by stretching a single colony indicates hypermucoviscosity . 2. Capsule Staining: to visualize large capsules . 3. Siderophore Production: Assessed on chrome azurol S (CAS) agar to detect iron-chelating activity. 7. Detection of siderophore genes by Simple qualitative PCR .
Key facts
- Study ID
- NCT07475429
- Run by
- Sohag University
- People needed
- 100
- Starts
- 2026-03-15
- Expected to finish
- 2026-10-01
- Last updated by the study team
- 2026-03-24
Who can join
Age: any. Sex: any. Healthy volunteers: accepted.
You may qualify if…
- 1. Patients with hypervirulant K. pneumoniae infection from various clinical specimens (blood, urine, sputum, wound swabs, etc.) Confirmed by culture and initial laboratory testing .
- Age and sex range: any age and any sex.
You may not qualify if…
- Patients who received antibiotic treatment in the 48 hours prior to sample collection.
- Patients with incomplete clinical data or inadequate samples.
Where it is running
- Faculty of Medicine — Sohag, Egypt
Full record on ClinicalTrials.gov
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