Effects of Propolis Flavonoids on TLR4 Signaling and Inflammation in Human PDLSCs In Vitro

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Conditions studied: Inflammatory Cytokine Expression

In brief

This in-vitro study evaluated the effects of a flavonoid-rich propolis extract on Toll-like receptor 4 (TLR4) signaling and inflammatory cytokine expression in human periodontal ligament stem cells (PDLSCs). PDLSCs were treated with propolis flavonoids under baseline conditions and following lipopolysaccharide (LPS)-induced inflammation, with untreated cells serving as controls. TLR4 gene expression was analyzed using PCR, while levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) were quantified by ELISA. Cell viability and cytotoxicity at different extract concentrations were assessed using the MTT assay. The study aimed to determine the immunomodulatory and cytocompatible potential of propolis flavonoids in regulating inflammatory responses in PDLSCs.

Key facts

Study ID
NCT07367451
Run by
Cairo University
People needed
20
Starts
2026-01-25
Expected to finish
2026-02-10
Last updated by the study team
2026-02-05

Who can join

Age: any. Sex: any. Healthy volunteers: not accepted.

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Full record on ClinicalTrials.gov

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