Validating IAAO for Muscle Outcomes

Recruiting now · Not applicable

Conditions studied: Protein Metabolism

In brief

Consuming dietary protein stimulates whole-body and muscle protein synthesis, the latter of which is typically measured using invasive primed constant infusions of stable isotopes with concurrent muscle biopsies. Alternative non-invasive methodologies have been developed (namely the indicator amino acid oxidation (IAAO) technique) to estimate the impact of protein ingestion on whole-body protein synthesis as a proxy for determining dietary protein requirements. Given that the IAAO technique is based on principles of protein metabolism which occur in the liver, it is unclear how representative the IAAO outcomes of whole-body protein synthesis is to skeletal muscle protein synthesis. Validation of the IAAO technique against gold-standard, biopsy-derived measures of muscle metabolism (i.e., muscle protein synthesis) would assist in mitigating the invasiveness of muscle physiology and nutrition research.

Key facts

Study ID

NCT07317921

Run by

University of Toronto

People needed

8

Starts

2025-12-01

Expected to finish

2026-05-20

Last updated by the study team

2026-01-05

Who can join

Age: 18 and older, up to 35. Sex: any. Healthy volunteers: accepted.

You may qualify if…

• Participants will be aged 18-35 years old
• Participants will have a BMI within the normal range (i.e., 18.5-24.9) and waist-to-hip circumference ratio of <0.95 for males and <0.8 for circumference for females to ensure homogeneity of the sample population
• Participants are willing to abide by the compliance rules of this study (e.g., abstain from extraneous physical activity 48h prior to session 4
• Self-reported regular menstrual cycle (25-35d) within the last 3 months (female participants)
• Physical activity score of ≥24 units as measured by the Godin leisure time exercise questionnaire

You may not qualify if…

• Inability to adhere to any of the compliance rules judged by principal investigator
• Self-reported regular tobacco or illicit drug use (e.g., growth hormone or testosterone)
• Current use of hormonal contraceptives
• Individuals with a history of allergy to local anesthetics (e.g., lidocaine)

Where it is running

• Kinesiology & Physical Education — Toronto, Ontario, Canada (enrolling)

Full record on ClinicalTrials.gov

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