Impact of Guided Biofilm Therapy on Epithelial and Mesenchymal Biomarkers in Periodontitis Patients

Recruiting now · Not applicable

Conditions studied: Periodontitis

In brief

The goal of this clinical trial is to investigate the clinical and biochemical effects of the guided biofilm therapy protocol as an alternative to the conventional non-surgical periodontal therapy in patients with periodontitis. The main question it aims to answer is: Does the guided biofilm therapy (GBT) protocol have an impact on gingival crevicular fluid (GCF) epithelial and mesenchymal biomarkers in patients with periodontitis? All patients will be instructed to brush their teeth twice daily and will be supplied with the same type of toothpaste and toothbrush, with suitable interdental aids. All patients will be instructed to attend again after 7 days. At baseline, full-mouth periodontal charting and radiographs will be obtained and after 1-hour, GCF samples will be collected from both test and control groups. Sites allocated to control group will undergo supragingival debridement by using ultrasonic device. After one week, the control group will receive RSD using area specific (Gracey) curettes (Medesy, Italy) according to the 24-hours protocol and will receive teeth polishing by using rubber polishing cups and tooth polishing paste, while the test group will receive a full GBT protocol which includes eight steps: (1) ASSESS, (2) DISCLOSE, (3) MOTIVATE, (4) AIRFLOW, (5) PERIOFLOW, (6) PIEZON PS\*, (7) CHECK, and (8) RECALL. After finishing the treatment session, the patients will be asked to attend for follow up, clinical evaluation of PI, BOP, PPD, and CAL, and GCF samples collection after 1 month and 3 months.

Key facts

Study ID: NCT07286916
Run by: University of Baghdad
People needed: 18
Starts: 2026-01-04
Expected to finish: 2026-06-01
Last updated by the study team: 2026-02-27

Who can join

Age: 18 and older. Sex: any. Healthy volunteers: not accepted.

You may qualify if…

Adult subjects (>18 years).
Subjects are not currently under active periodontal therapy or joining other trial in the last 3 months.
Patients must be diagnosed with generalized unstable periodontitis with bilateral, symmetrical distribution of PPD ≥ 4mm and positive BOP.

You may not qualify if…

Patients not diagnosed with periodontitis.
Patients consuming antibiotics.
Regular users of nonsteroidal anti-inflammatory drugs (NSAIDs).
Patients receiving periodontal treatment 3-months prior to the study.
Pregnant or mothers in a breastfeeding period.
Third molars and teeth with furcation involvement.
Patients taking antioxidant supplements.
Patients with heavy calculus that would prevent PerioPaper strips entrance to the periodontal pocket.
Patient with pulmonary diseases and with a history of allergy to erythritol.
Patients not willing to participate. -

Where it is running

College of Dentistry, University of Baghdad — Baghdad, Iraq (enrolling)

Full record on ClinicalTrials.gov

Trial information comes from ClinicalTrials.gov and is refreshed daily. TrialsForMe does not provide medical care and does not run the studies it lists.