Methylene Blue Treatment of Chronic Hepatitis B Virus Infection

Recruiting now · Phase 2

Conditions studied: Chronic HBV Infection

In brief

HBV infection is a global public health problem. It is estimated that there are more than 250 million HBV carriers worldwide, of whom approximately 600,000 die each year from HBV-related liver disease. Diagnosis and treatment depend on the stage of infection, the degree of liver inflammation, and the progression of fibrosis. Patients with HBeAg-positive or -negative hepatitis are usually treated with nucleos(-t)ide analogues. This treatment inhibits HBV reverse transcriptase and reduces viral replication, but does not affect HBsAg production. Treatment must be continued for life in most cases and is associated with high cumulative costs and the risk of development of resistance. Recently, it has been shown that patients who show a decrease in their HBsAg levels are more likely to become HBsAg negative, seroconvert and be able to stop antiviral treatment, which is the ultimate goal of treatment, but unfortunately rarely achieved. Methylene blue (MB) has been shown to have an effect on HBsAg levels in vitro, making it a promising test for a new treatment for HBV. This is an explorative pilot study, open label, dose-escalation, single center clinical trial on the efficacy of MB against HBV infection. Primary objective is to assess the efficacy of incremental doses of MB against hepatitis B virus in patients with chronic hepatitis B assessed by the reduction of both the HBsAg and HBV DNA levels after 6 months of treatment and 6 months of follow-up. Participant will be treated for 6 months with MB 100 mg capsules, and then followed up for a further 6 months. The initial dose of MB might be increased if the patient is a non-responder.

Key facts

Study ID: NCT06887036
Run by: Fondazione Epatocentro Ticino
People needed: 10
Starts: 2025-09-01
Expected to finish: 2026-10-31
Last updated by the study team: 2025-08-17

Who can join

Age: 18 and older, up to 79. Sex: any. Healthy volunteers: not accepted.

You may qualify if…

Signed informed consent
Age ≥ 18 and < 80 years
Microbiologically confirmed chronic Hepatitis B Virus infection (according to the old terminology "inactive HBV carrier")
HBsAg levels within 20-15'000 IU/ml measured at the at least twice at 6-18 months apart
HBV DNA PCR levels within 1'000-20'000 IU/ml measured at least twice at 6-18 months apart
Negative pregnancy test in women of child-bearing age

You may not qualify if…

Documented refusal to participate in the study
Known G-6-Phophatase deficiency
Treatment with a serotoninergic drug
Ongoing treatment with a nucleos(-t)ide treatment
Clinically relevant concomitant liver disease
GPT > 2xULN
Fibroscan of > 8.0 KPa obtained ≤ 12 months before Visit 0/Screening
HBeAg positivity
Anti HDV antibody positivity
Breastfeeding women

Where it is running

Epatocentro Ticino — Lugano, Canton Ticino, Switzerland (enrolling)

Full record on ClinicalTrials.gov

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