PRObing The Efficacy of Commercial Stage Storage Buffers and Evaluating Gut Metaproteome Variability Between Individuals
Recruiting now
Conditions studied: Gut Microbiome, Buffer, Methodology Study, Fecal Microbiota, Variability
In brief
The study aims to assess the effectiveness of various commercial and non-hazardous buffers for the storage of human gut fecal samples over time. This evaluation will be conducted by comparing the performance of these buffers against directly frozen samples using metaproteomic analysis. The study is motivated by the need for standardized protocols for sample preservation in metaproteomic research, particularly focusing on protein preservation in fecal samples. By investigating proteomic, taxonomic, and functional identifications, the research seeks to provide insights into the reliability of these buffers as storage solutions. Additionally, the study plans to explore inter- and intra-individual variabilities at the proteome level by periodically collecting fecal samples from volunteers, complementing existing knowledge in metaproteomics. Overall, the study addresses a critical gap in the field and has the potential to enhance reproducibility and comparability across metaproteomic studies
Key facts
- Study ID
- NCT06423508
- Run by
- University of Vienna
- People needed
- 52
- Starts
- 2024-12-03
- Expected to finish
- 2025-09-30
- Last updated by the study team
- 2025-03-25
Who can join
Age: 18 and older, up to 70. Sex: any. Healthy volunteers: accepted.
You may qualify if…
- agreeing to participate after signing the informed consent form and
- being 18-70 years old.
You may not qualify if…
- take antibiotics during the previous 1 month
- had undergone gastrointestinal surgery
Where it is running
- UVienna — Vienna, State of Vienna, Austria (enrolling)
Full record on ClinicalTrials.gov
Trial information comes from ClinicalTrials.gov and is refreshed daily. TrialsForMe does not provide medical care and does not run the studies it lists.