To Evaluate the Safety and Efficacy of ADP-TILIL7 in Patients With Locally Advanced or Metastatic Melanoma

Recruiting now · Phase 1

Conditions studied: Melanoma Stage III, Melanoma Stage IV, Melanoma

In brief

The primary objective of this Phase 1 clinical trial is to evaluate the feasibility and tolerability of a novel generation of gene-modified tumor infiltrating lymphocytes (TILs) in a cohort of 10 patients aged 18-75 diagnosed with unresectable or metastatic melanoma. TILs will undergo transduction with the Interleukin-7 (IL-7) gene, for IL-7 production upon antigen engagement. Participants will undergo: * screening * tumor operation following autologous TIL production (incl. transduction) - takes approximately 4-6 weeks * admission for lymphodepleting chemotherapy (Cyclophosphamide and Fludarabine phosphate), TIL infusion and high-dose IL-2 infusions for a maximum of 6 doses * Following treatment, patients will undergo systematic and regularly planned assessments, encompassing clinical evaluation, biochemistry analyses, and PET/CT scans. This thorough follow-up regimen will be continued until any of the following events occur: progressive disease, withdrawal from study, or end of study, which spans a duration of 15 years for trials involving genetically modified organisms.

Key facts

Study ID

NCT06204991

Run by

Inge Marie Svane

People needed

10

Starts

2025-04-01

Expected to finish

2028-04-01

Last updated by the study team

2025-08-24

Who can join

Age: 18 and older, up to 75. Sex: any. Healthy volunteers: not accepted.

You may qualify if…

• All the criteria listed in the following need to be met before patient inclusion.
• Histologically confirmed inoperable or metastatic melanoma (stage IIIc or IV).
• Progressive disease after standard treatment with PD-1 check-point inhibition or combination of aforementioned with CTLA-4 check-point inhibition.
• Age: 18 - 75 years at time of signed Informed consent.
• ECOG performance status of ≤ 1 (Appendix 2).
• Is fit for tumor resection and has at least one lesion (> 1 cm3) available for surgical resection for manufacture of TIL.
• At least one measurable parameter in accordance with RECIST 1.1 -criteria (excluding lesion to be resected).
• LVEF assessment with documented LVEF ≥50% by either TTE (transthoracic echocardiography) or MUGA (multigated acquisition scan).
• Sufficient organ function, including:
• Absolute neutrophil count (ANC) ≥ 1.500 /µl
• Leucocyte count ≥ lower normal limit
• Platelets ≥ 100.000 /µl and <700.000 /µl
• Hemoglobin ≥ 6.0 mmol/l
• eGFR > 70 ml/min*
• S-bilirubin ≤ 1.5 times upper normal limit (Exception: Subjects with liver metastasis ≤ 2.5 × ULN)
• ASAT/ALAT ≤ 2.5 times upper normal limit (Exception: Subjects with liver metastasis ≤ 5.0 × ULN)
• Alkaline phosphatase ≤ 5 times upper normal limit
• Lactate dehydrogenase ≤ 5 times upper normal limit
• Sufficient coagulation: APPT<40 and INR<1.5
• * In selected cases it can be decided to include a patient with an eGFR < 70 ml/min with the use of a reduced dose of chemotherapy.
• Signed statement of consent after receiving oral and written study information 10. Willingness to participate in the planned controls and capable of handling toxicities.
• Subject must receive T-cell therapy as the next therapy following tumor resection, unless bridging therapy is administered:
• Bridging therapy is discouraged. However, if in the opinion of the Investigator, the subject requires immediate therapy after tumor resection, the subject may receive bridging therapy for the period during which the subject is awaiting the manufacture of TIL-infusion product. Bridging therapy may be a continuation of the therapy the subject was receiving prior to tumor resection or may be a new therapy.
• Following this bridging therapy, the subject must adhere to the mandatory washout periods (exclusion criterion 1) and must continue to have measurable disease prior to receiving T-cell therapy.
• Age and Reproductive Status:

You may not qualify if…

• Patients will be excluded if they meet one of the criteria's listed below
• Subject has received or plans to receive the following therapy/treatment prior to tumor resection (TR) or lymphodepleting chemotherapy (LDC):
• Cytotoxic chemotherapy: Washout period 3 weeks before TR and LDC
• Small molecules/TKI: Washout period 1 week before TR and LDC
• Immune therapy (monoclonal AB therapy, CPI and biologics): 2 weeks before TR and LDC
• Prior T-cell therapy, including gene therapy using an integrating vector
• Corticosteroids at dose equivalent > 10 mg prednisone or any other immunosuppressive therapy. 2 weeks before TR and LDC. Note: Use of topical steroids is not an exclusion
• Investigational treatment: 4 weeks or 5 half-lives, whichever is shorter before TR and LDC
• Radiation to the pelvis and/or multiple bones containing ≥ 25% of bone marrow: 4 weeks before TR and LDC
• Whole brain radiotherapy or brain stereotactic radiosurgery: 4 weeks before TR and LDC
• Radiotherapy to the target lesions: 3 months prior to TIL-infusion. A lesion with unequivocal progression post-radiotherapy may be considered a target lesion.
• A history of prior malignancies. Patients treated for another malignancy can participate if they are without signs of disease for a minimum of 2 years after treatment. Subjects with curatively treated ductal carcinoma in situ (DCIS or LCIS) breast cancer for which they are taking hormonal therapy is acceptable. Resectable squamous or basal cell carcinoma of the skin is acceptable.
• Patients with metastatic ocular/mucosal or other non-cutaneous melanoma. Unknown primary melanoma is eligible.
• Toxicity from previous anti-cancer therapy must have resolved to ≤ Grade 1 or baseline prior to enrollment (except for non-clinically significant toxicities e.g., alopecia, vitiligo).
• Subjects with Grade 2 toxicities who are deemed stable or irreversible (e.g., peripheral neuropathy) can be enrolled.
• Patients who have more than 2 CNS metastases or who have any CNS lesion that is symptomatic, greater than 1 cm in diameter or show significant surrounding edema on MRI scan will not be eligible until they have been treated and demonstrated no clincal or radiologic CNS progression for at least 2 months.
• The following patients will be excluded because of inability to receive high dose interleukin-2:
• History of coronary revascularization
• Patients with clinically significant atrial and/or ventricular arrhythmias including but not limited to: atrial fibrillation, ventricular tachycardia, 2o or 3o heart block
• Documented FEV-1 less than or equal to 60% predicted value for patients with: A prolonged history of cigarette smoking (greater than 20 pack years), large tumor burden in the lungs or Symptoms of respiratory distress.
• Known hypersensitivity to one of the active drugs or one or more of the excipients.
• Severe medical conditions, such as severe asthma/COLD, significant cardiac disease, poorly regulated insulin dependent diabetes mellitus among others.
• Acute/chronic infection with HIV, hepatitis, syphilis among others.
• Severe allergies or previous anaphylactic reactions.
• Active autoimmune or immune-mediated disease that has not yet resolved. Subjects with the following will be eligible:

Where it is running

• Department of Oncology — Herlev, Denmark (enrolling)
• Department of Oncology — Herlev, Denmark (enrolling)

Full record on ClinicalTrials.gov

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