Developing a Pipeline to Employ RNA-Seq as a Complementary Diagnostic Tool in Rare Diseases

Recruiting now · Not applicable

Conditions studied: Atypical Hemolytic Uremic Syndrome, Membranoproliferative Glomerulonephritis, Autosomal Dominant Polycystic Kidney, Healthy

In brief

This project aims to identify, through RNA-Seq technology, the genetic alterations underlying undiagnosed rare diseases in pediatric and adult patients with early onset and with negative WES. * Objective 1: Set up and validate techniques. Set-up and validation of the transcriptome analysis protocol in healthy subjects and in patients with known splicing alterations and/or altered RNA expression. * Objective 2: Diagnostic phase. Study of splicing alterations and RNA levels in cultured fibroblasts obtained from skin biopsies of patients with rare genetic diseases and negative exome. Exploratory goals * Compare the RNA expression profile obtained from skin biopsy-derived fibroblasts with the RNA expression profile from blood. The most relevant results will be validated in qRT-PCR. * To analyze the transcriptional and protein profile heterogeneity in skin-derived fibroblasts in enrolled subjects. To explore the effects of genetic (from WES) and transcriptional (from RNA-seq) alterations in participants' plasma and serum. Healthy controls Five healthy subjects will be recruited from the staff of the Mario Negri Institute for Pharmacological Research. The coded samples will be used to set up the method of isolation and culture of skin fibroblasts and RNA-Seq. Validation group For the set-up and validation of the skin fibroblast isolation and RNA-Seq procedure, ten adult patients with known diagnosis and with alterations in RNA levels and/or splicing will be recruited as positive controls. Patients who meet the requirements described above will be contacted by the doctors of the Daccò Center for an interview explaining the project. Those who agree to participate in the study will be asked to sign the informed consent before proceeding with the experimental part. "Discovery/Exploration" group The exploration cohort will be composed of 30 symptomatic undiagnosed patients with suspected genetic disease (children and adults with infantile onset) belonging to the Clinical Center of the Mario Negri Institute for Pharmacological Research and for whom WES investigations did not reveal causative genetic alterations.

Key facts

Study ID

NCT05996731

Run by

Mario Negri Institute for Pharmacological Research

People needed

105

Starts

2024-02-21

Expected to finish

2026-06-01

Last updated by the study team

2026-03-23

Who can join

Age: any. Sex: any. Healthy volunteers: accepted.

You may qualify if…

• Male and female adults
• Written informed consent

You may not qualify if…

• Inability to understand the potential risk and benefits of the study
• Legal incapacity
• Validation cohort.
• Inclusion criteria:
• Male and female adults
• Genetic diseases affecting RNA levels (frameshifts, stop, large deletions, alteration of canonical splicing sites)
• Written informed consent
• Exclusion criteria:
• Underage patients
• Inability to understand the potential risk and benefits of the study
• Legal incapacity
• Discovery cohort.
• Inclusion criteria:
• Male and female patients (children and adults with onset in infancy or early adulthood) with rare genetic undiagnosed diseases
• Patients with no strong candidates based on previous genetic analysis such as WES, but with clinically suspicion of a genetic rare disease
• Written informed consent
• Exclusion criteria:
• Inability to understand the potential risk and benefits of the study
• Legal incapacity

Where it is running

• Centro di Ricerche Cliniche per le Malattie Rare "Aldo e Cele Daccò" — Ranica, BG, Italy (enrolling)

Full record on ClinicalTrials.gov

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