Phase I Study of CTL Anti-DP Infusion Post-hematopoietic Stem Cell Transplantation
Recruiting now · Phase 1
Conditions studied: Haematologic Disease
In brief
For several decades, allogeneic hematopoietic stem cell trans-plantation (allo-HSCT) has remained an important strategy in the management of patients with high-risk hematological malignancies. The acceptance of umbilical cord blood (UCBT) and haploidentical grafts (Haplo) as viable alternative donors for allo-HSCT has increased the options for patients with no matched donors and now ensures that a donor can be identified for virtually all patients. Relapsed disease is a principal threat to these patients and affects 30-50% of them. The therapeutic options for these relapsing patients are diverse but remain largely ineffective in altering their long-term outcomes. Therefore, pre-emptive treatment post allo-HSCT is considered. MHC (major histocompatibility complex) class II molecules are a family of molecules normally found only on hematopoietic cells. cell-surface proteins are responsible for the regulation of the immune system in humans and are important in disease defense. They are the major cause of organ transplant rejections. Different HLA-DPB1 alleles exist in the general population. HLA-DPB1\*04:01 is the most frequent (70.5%) while HLA-DPB1\*02:01 represents 32% and HLA-DPB1\*03:01 20%. In allo-HSCT, the donor and the recipient may express different HLA-DPB1 molecules. HLA-DPB1 matching status has an impact on GVL (graft versus leukemia) and GVHD. In recipients of HSCT, a match for DPB1 is associated with a significantly increased risk of disease relapse, irrespective of the matching status of other HLA molecules.. Therefore, one could anticipate that a mismatched of HLA class II could induce a selective GVL reactivity without GVHD. HLA-DP-expressing B cell and myeloid malignancies can be recognized and lysed by HLA-DP-specific T cells. The majority of leukemic cells (Acute Myeloid Leukemia, Acute Lymphoid Leukemia, Chronic Lymphoid Leukemia) express HLA-DP. A T cell clone recognizing specifically HLA-DPB1\*0401 has been developed as a permanent cell line This clone has been demonstrated to be able to kill HLA-DPB1\*0401 positive leukemic cells. In addition, this clone harbors a special suicide gene allowing the destruction of the clone in presence of a specific anti-viral drug named ganciclovir. We hypothesize that infusion of a third party suicide gene-transduced T cell clone directed against HLA-DPB1\*401 might protect against possible relapse of hematological malignancies. We propose to inject iv escalating dose of a third party clone recognizing HLA-DPB1\*04:01, 4 to 5 months following transplantation (when immunosuppressive drugs have been discontinued) in patients HLA-DPB1\*04:01 positive with a donor HLA-DPB1\*04:01 negative to evaluate the feasibility, toxicity, benefits of this immune intervention.
Key facts
- Study ID
- NCT04180059
- Run by
- Nantes University Hospital
- People needed
- 6
- Starts
- 2020-02-09
- Expected to finish
- 2027-08-01
- Last updated by the study team
- 2026-01-26
Who can join
Age: 18 and older, up to 75. Sex: any. Healthy volunteers: not accepted.
You may qualify if…
- Patients HLA-DPB1*04:01 positive, with confirmed diagnosis of hematologic malignancies (AML, Myelodysplasic and myeloproliferative syndrome, ALL, non-Hodgkin's lymphoma, Hodgkin's disease, CLL), undergoing an allo-HSCT using a HLA-DPB1*04:01 negative donor.
- The graft can be PBSC (peripheric blood stem cells) or bone marrow.
- Patients aged between 18-75 years.
- Patients in complete remission or >50% of response (for lymphoma) at time of transplant.
- have a donor with no contra-indications for mobilization of peripheral blood stem cells using G-CSF (colony-stimulating factors)
- Affiliation number to the National Health Care System
- Lack of reactivity of the clone against the donor's cells (PHA-blasts prepared for from PBMCs).
- For cord blood transplants: cord blood must be HLA-DPB1*04:01 negative and the HLA compatibility (A, B, DR) between the cord blood and the recipient must be 4/6, 5/6 or 6/6.
- ECOG <=2 or Karnofsky >60%
- neutrophils ≥ 1 000 cells /μl and/or platelets ≥ 50 000 cells/μl (growth factor allowed)
You may not qualify if…
- pregnant or breastfeeding woman
- patient refusing contraception measure
- minor
- Adult patients under guardianship, curatorship or justice protection
- Patients with post-transplant relapse within the clone injection time (before D100)
- Karnofsky performance score below 60%or ECOG >2
- Acute and chronic heart failure (NYHA Class III or IV) or symptomatic ischemic heart disease.
- Severe liver failure (bilirubin >30 µmoles/L, SGPT (Serum Glutamo-Oxalacetic Transaminase)> 4 X upper limit of normal).
- Impaired renal function (creatinine clearance < 30 ml/min)
- Acute GVHD > grade 1
- Active uncontrolled infection.
- Denied to provide informed consent
- Severe neurological or psychiatric disorders as determined by the study physician.
- Treatment with other investigational drugs following allogeneic transplantation.
Where it is running
- Chu de Nantes — Nantes, France (enrolling)
Full record on ClinicalTrials.gov
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