Effect of SB CD on Chylomicron

Completed

Conditions studied: Crohn Disease

In brief

It is often suggested that lymphatic vessels are ineffective in transporting cargo in Crohn's disease. Our own work on surgically resected tissue supports this concept (1), but the concept has not been directly tested. Chylomicrons are packaged lipids from the diet with an obligatory absorption route through the lymphatic vasculature to reach host plasma. This protocol takes an approach to directly quantify chylomicron secretion using a fatty meal that incorporates stable isotopic tracers for trioleate and cholesterol in the meal. We will collect baseline plasma and then plasma every 30 minutes for 6 hours to chart the kinetic and magnitude of chylomicron secretion and transport in all subjects using mass spectrometry analysis. We will characterize a wide variety of parameters on the chylomicrons as well using ELISA. Infusions i.v. of stable isotope labeled triglyceride and glycerol will allow us to consider whether there are changes in VLDL metabolism that could account for differences in chylomicron handling once the chylomicrons are secreted into plasma. Collection and analysis of breath samples will also be carried to normalize against possible incomplete lipid absorption in some subjects.

Key facts

Study ID

NCT03496818

Run by

Washington University School of Medicine

People needed

47

Starts

2018-04-16

Expected to finish

2020-06-01

Last updated by the study team

2020-12-03

Who can join

Age: 18 and older, up to 80. Sex: any. Healthy volunteers: accepted.

You may not qualify if…

• A history of diabetes mellitus.
• A history of hyperlipidemia.
• A history of thyroid dysfunction.
• A history of hepatic, cardiac, renal or endocrine dysfunction.
• A history of cancer requiring systemic chemotherapy
• Prior radiation therapy to pelvis or abdomen
• Taking medications (current or past 30 days) known to alter plasma lipid levels including statins, corticosteroids (oral, IV or topical), bile acid sequestrants, thyroid drugs, or fibrates.
• Currently pregnant or trying to become pregnant.
• History of abdominal or pelvic surgery.
• Complicated small bowel CD with obstruction, penetrating disease and/or abscess.
• Recent surgery of any type within the last 30 days that, in the discretion of the principal investigator, might affect the study or pose risk to the subject
• Exclusion for control group: all of the above and no recent participation in other studies as a control.

Where it is running

• Washington University School of Medicine — St Louis, Missouri, United States

Full record on ClinicalTrials.gov

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