Intracranial Injection of NK-92/5.28.z Cells in Combination With Intravenous Ezabenlimab in Patients With Recurrent HER2-positive Glioblastoma

Recruiting now · Phase 1

Conditions studied: Glioblastoma

In brief

The main objective of this clinical study is to evaluate the safety and tolerability of NK-92/5.28.z and to determine the maximum tolerated dose or maximum feasible dose (MFD). Recommended phase 2 doses both for intraoperative injections only (RP2Diio) and repetitive injections (RP2Dri) will be determined. Frequent side effects and target organs of toxicity and their severity, duration and reversibility will be determined. Furthermore, pharmacokinetics and pharmacodynamics will be examined. In addition, potential signs of anti-tumor activity of NK-92/5.28.z cells will be analyzed. In the separate "CAR2BRAIN-Check" and "CAR2BRAIN-CheckR" cohorts, combination therapy of NK-92/5.28.z with the anti-PD-1 antibody Ezabenlimab (BI 754091) will be tested.

Key facts

Study ID: NCT03383978
Run by: Johann Wolfgang Goethe University Hospital
People needed: 42
Starts: 2017-12-01
Expected to finish: 2028-06-30
Last updated by the study team: 2026-04-23

Who can join

Age: 18 and older. Sex: any. Healthy volunteers: not accepted.

You may qualify if…

Recurrent or refractory HER2-positive glioblastoma or its variant gliosarcoma in which relapse surgery (partial or total) or a biopsy (biopsy only for the "CAR2BRAIN-CheckR" cohort) is being planned. Those patients with planned biopsy may be included into the "CAR2BRAIN-CheckR" cohort, if all of the following conditions apply:
Biopsy is necessary (as determined by the treating physician) to rule out the differential diagnosis of pseudoprogression prior to relapse surgery.
Patients must be candidates for relapse surgery, which must be postponable for four weeks.
Prior therapy must include the standard of care for glioblastoma (radiotherapy and alkylating chemotherapy, or at least a part thereof if the therapy was terminated prematurely due to therapy failure or poor tolerance). For patients with non-methylated MGMT-Promotor, prior alkylating chemotherapy is dispensable.
Age ≥ 18 years
Life expectancy ≥ 3 months
Bilirubin ≤ 3x normal, AST ≤ 5x normal, ALT ≤ 5x normal, serum creatinine ≤ 2x upper limit of normal for age, leukocyte count ≥ 3/nl, thrombocyte count ≥ 100/nl and Hb ≥ 8.0 g/dl
Blood oxygenation of ≥ 90% as measured by pulse oximetry on room air
Women must have a negative serum pregnancy test within 72h prior to the start of the first NK-92/5.28.z cell injection.
Sexually active patients must be willing to utilize effective birth control methods throughout the study and for 24 weeks after the last NK-92/5.28.z cell injection. This includes two different forms of effective contraception (e.g. hormonal contraceptive and condom, IUD/IUS and condom) or sterilization.
Patients should have been off other antineoplastic therapy for two weeks prior to entry in this study. Temozolomide will be allowed up to 48h preinjection. At the time of inclusion, dexamethasone up to a total dose of 4 mg per day will be allowed if medically indicated.
Informed consent explained to and signed by patient; patient given copy of informed consent.
Karnofsky performance score of ≥ 70%

You may not qualify if…

Anti-angiogenic therapy e.g. with bevacizumab in the last four weeks prior to study entry
Previous anti-PD-1 or anti-PD-L1 directed checkpoint inhibitor therapy (only "CAR2BRAIN-Check" cohort)
Coagulation disorder (INR>1.4 or PTT>50sec) or anticoagulation in therapeutic dosage
History of autoimmune disease, including but not limited to myasthenia gravis, myositis, autoimmune hepatitis, systemic lupus erythematosus, rheumatoid arthritis, inflammatory bowel disease, vascular thrombosis associated with antiphospholipid syndrome, Wegener's granulomatosis, Sjögren's syndrome, Guillain-Barré syndrome, multiple sclerosis, vasculitis, or glomerulonephritis. However, patients with a history of autoimmune-related hypothyroidism on a stable dose of thyroid replacement hormone may be eligible for this study.
Patients with Type I diabetes mellitus not on a stable dose of insulin regimen
Psoriatic arthritis (however, patients with eczema, psoriasis, lichen simplex chronicus, or vitiligo with dermatologic manifestations only are permitted provided that they meet all of the following conditions:
Rash must cover less than 10% of body surface area
Disease is well controlled at baseline and only requiring low potency topical steroids
No acute exacerbations of underlying condition within the previous 12 months (not requiring psoralen plus ultraviolet A radiation, methotrexate, retinoids, biologic agents, oral calcineurin inhibitors, high potency or oral steroids))
Patients with clinical or laboratory signs for immunodeficiency or under immunosuppressive medication other than corticosteroids
Severe intercurrent infection
Known HIV, HBV (defined by detection of HBsAg) or HCV positivity (defined by detection of HCV-IgG)
Chronic heart failure NYHA ≥III
Patients with a prior solid organ transplantation or allogenic haematopoietic stem cell transplantation
Patients unable to undergo MRI
Pregnancy or breastfeeding
Drug or alcohol abuse
Severe psychiatric disorder which might interfere with the study treatment or examination
Simultaneous participation in another interventional clinical trial. If a subject participated in a trial testing another IMP, such IMP should have been terminated at least 30 days before inclusion of the subject.

Where it is running

Neurochirurgische Klinik, Universitätsklinikum Heidelberg — Heidelberg, Baden-Wurttemberg, Germany (enrolling)
Neurochirurgische Klinik, Universitätsmedizin Mannheim — Mannheim, Baden-Wurttemberg, Germany (enrolling)
Neurochirurgische Klinik, Universitätsmedizin Mainz — Mainz, Rhineland-Palatinate, Germany (enrolling)
Johann W. Goethe University Hospital, Department of Neurosurgery — Frankfurt, Germany (enrolling)
Johann W. Goethe University Hospital, Senckenberg Institute of Neurooncology — Frankfurt, Germany (enrolling)

Full record on ClinicalTrials.gov

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