Assessment of Intrahepatic Hepatitis C Virus (HCV) RNA Levels at the Time of Liver Transplantation

Status unconfirmed

Conditions studied: Hepatitis C Virus, Liver Transplantation

In brief

The purpose of this study is to measure intrahepatic HCV RNA levels at the time of liver transplantation in patients receiving antiviral therapy while on the liver transplant waiting list. This will eventually be correlated with the degree of hepatic fibrosis present within different geographic sites in the cirrhotic liver. Tissue samples will be obtained from the patient's liver explant as well as hilar lymph nodes. Upon the removal of the cirrhotic liver at the time of transplantation, the explant will be biopsied multiple times in different segments of the liver and preserved for viral detection studies as well as analysis of the degree of fibrosis. Peripheral blood mononuclear cells (PBMCs) will be obtained for viral detection at the time of transplantation. Serum HCV RNA levels will also be obtained at 1 month, 3 months and 6 months post liver transplantation. Study Hypotheses: * Virological relapse or non-response is higher is patients with cirrhosis due to failure of antiviral medication to concentrate adequately in a fibrotic liver having an altered sinusoidal micro-architecture * HCV may persist in different geographic regions of the fibrotic liver in part predicated on blood supply to that area and this may have an effect on overall virological response. These differences in viral persistence and detection may exist in different lobes of the liver or even within a few centimeters within the same portion of the liver parenchyma. * PBMC and hilar lymph nodes may be extrahepatic reservoirs of HCV viral persistence in patients receiving antiviral therapy and may account for virological relapse post-therapy * There may be varying degrees of fibrosis within the same cirrhotic liver which may impact on hepatic synthetic function and antiviral response to treatment.

Key facts

Study ID

NCT01836718

Run by

Schiano, Thomas D., MD

People needed

20

Starts

2013-06-01

Expected to finish

2018-06-01

Last updated by the study team

2017-01-30

Who can join

Age: 18 and older, up to 80. Sex: any. Healthy volunteers: not accepted.

You may qualify if…

• Patients age 18-80 with chronic HCV on the liver transplant waiting list may be eligible for participation
• The following subjects will be enrolled:
• Patients undergoing liver transplantation who are documented HCV viral load undetectable while on antiviral therapy
• Patients receiving anti-viral therapy and who have a detectable HCV viral load
• Patients not currently receiving antiviral therapy and are HCV PCR (+) will be included and serve as a comparison group

You may not qualify if…

• Patients who are co-infected with HIV and/or HBV will not be included

Where it is running

• Mount Sinai School of Medicine Recanati/Miller Transplantation Institute — New York, New York, United States

Full record on ClinicalTrials.gov

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