Identification of Muscle-specific Biomarkers of Fatty Acid Beta-oxidation

Completed · Not applicable

Conditions studied: Obesity

In brief

Elevated fat levels within skeletal muscle cells (intramyocellular lipids) are highly correlated with muscle and whole-body insulin resistance, and more prevalent in obesity. The molecular links and metabolic shifts driving this association remain open to debate, but notably, reduced muscle mitochondrial fatty acid (FA) ß-oxidation is more prevalent among insulin-resistant/diabetic persons. Therefore, discovery of biomarkers reflective of the status of an individual's muscle FA ß-oxidation activity or capacity would have tremendous prognostic and diagnostic value in terms of diabetes. Furthermore, characterization of metabolites associated with muscle mitochondrial fat metabolism should uncover candidate signaling factors which tie FA ß-oxidation to insulin signaling. The investigators propose to identify, for the first time, specific biomarkers of muscle FA ß-oxidation using multiple metabolomic analytical platforms to compare metabolite profiles in samples derived from biological systems displaying disparate muscle fat combustion. The current experiment will test whether plasma metabolites and/or metabolite signatures that track efficient muscular FA ß-oxidation can be experimentally increased in obese, insulin-resistant subjects via a diet-exercise regimen designed to improve muscle fitness and FA combustion.

Key facts

Study ID

NCT01494025

Run by

USDA, Western Human Nutrition Research Center

People needed

13

Starts

2009-10-01

Expected to finish

2012-07-01

Last updated by the study team

2021-07-07

Who can join

Age: 30 and older, up to 50. Sex: female. Healthy volunteers: accepted.

You may qualify if…

• female
• age 30-50
• menstruating with regularity
• BMI between 30- 37.5 with stable body weight (<5% change) over last 6 mos
• waist to hip ratio >0.9
• sedentary lifestyle (typical exercise < 30 min. per week)
• non-smoker
• evidence for pre-diabetes (hyperinsulinemia and/or blood glucose > 100 < 126 mg/dl)

You may not qualify if…

• any clinically significant abnormal laboratory values
• any clinical signs of infection (i.e., fever)
• any chronic diseases
• any personal history of cardiovascular disease
• clinically-significant elevation in blood pressure (≥130/85)
• diabetes or other chronic disease
• any regular medications or drug abuse
• pregnancy or lactation

Where it is running

• Western Human Nutrition Research Center — Davis, California, United States

Full record on ClinicalTrials.gov

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