Use of Biopsy Punch for Skin Biopsies for Fibroblasts Culturing

Completed

Conditions studied: Fibroblasts

In brief

Fibroblasts can be cultured from human skin biopsies using fetal bovine serum and artificial growth medium. Fibroblasts cultured in this way retain the genetic characteristics of their donor but can be used for experiments where study conditions are much more precisely controlled than they can be in in vivo studies. We plan to culture fibroblasts from Pima Indians for two purposes. Firstly, to look for an in vitro system to measure insulin resistance, the underlying abnormality in Pima diabetics. Secondly, to look for biochemical markers of diabetes or obesity in Pimas employing two dimensional electrophoresis techniques.

Key facts

Study ID

NCT00340912

Run by

National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)

People needed

500

Starts

1988-12-07

Last updated by the study team

2017-07-02

Who can join

Age: 18 and older, up to 50. Sex: any. Healthy volunteers: not accepted.

You may not qualify if…

• We will exclude any seriously ill individuals, any known to have a bleeding diathesis or to be immunosuppressed or subjects who are carriers of HIV or HBV.
• Children will be excluded, as the age range for this study's participants is 18-50.
• Pregnant women will be excluded on the basis of a urinary pregnancy test.
• All other subjects are potentially included.

Where it is running

• NIDDK, Phoenix — Phoenix, Arizona, United States

Full record on ClinicalTrials.gov

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